Studies on the Mechanism of I-methyl-4-piienyl-i,2,3,6-tetrahydropyridine Cytotoxicity in Isolated Hepatoctyes

Oxidative stress and covalent binding have been proposed as possible mechanisms involved in the cytotoxic effects of the parkinsonism-causing compound l-methyl-4-phenyl-I,2,3,6-tetrahydzopyridine (MPTP). However, the toxicity induced by MPTP in isolated fat hepatocytes seems to be relatively independent of oxygen ~adical-induced oxidative stress. Here we demonstrate that MPTP cytotoxicity is not potentiated by pretreatment with 1,3-bis(2-chJoroetbyl)-l-nitrosourea (BCNU), an inhibitor of g]utathione reductase, nor prevented by the antioxidant N,N'diphenyl-p-phenylenediamine (DPPD) or the iron-chelating agent desferrioxamine. Moreover, preincubation of hepatocytes with diethylmaleate to lower the level of intracellular reduced glutathione (to 20% ol the initial value) did not affect either the rate or extent of MPTP cytotoxicity. Thus, nucleophilic soluble thiols do not seem to play a protective role against MPTPinduced cell damage, in contrast to what one would have expected il covalent protein binding and oxidative stress were involved as toxic mechanisms. On the other hand, MPTP cytotoxicity was potentiated by pretreatment of hepatocytes with cytochrome P-450 inhibitors (e.g., SKF 525A and metyrapone) and a more rapid depletion of ATP was observed in these experimental conditions. We conclude that mitochondrial damage and subsequent ATP depletion are likely to play a critical role in the toxicity of MPTP to isolated hepatocytes and that the metabolism of MPTP via the cytochrome P-450 monooxygenase system can be considered to be a detoxifying pathway.